How To Store Mushroom Spores?

Mushrooms are a delicious and healthy addition to meals, and growing them at home can be a rewarding experience. However, to cultivate these fungi successfully, it is essential to know how to store mushroom spores. Whether you are a beginner or an experienced grower, proper storage techniques will ensure that your mushroom spores remain viable and ready for use.

Storing mushroom spores can seem like a daunting task, but it is relatively simple once you understand the basics. In this guide, we will go over the different methods of storing mushroom spores and provide you with tips to keep them healthy and ready for use whenever you need them. So, let’s dive in and learn how to store mushroom spores like a pro!

how to store mushroom spores?

How to Store Mushroom Spores: A Complete Guide

1. Understanding Mushroom Spores


Mushroom spores are tiny reproductive cells that are produced by the fungi, which eventually develop into mature mushrooms. They are important for the propagation of mushroom species and can be easily collected from mature mushrooms by using a spore print. These spores can be stored for long periods and used for cultivating mushrooms in the future.

When it comes to storing mushroom spores, it is important to know that these spores are very delicate and require proper handling and storage conditions. Exposure to moisture, heat, and light can damage spores and reduce their viability.

Benefits of Storing Mushroom Spores:


– Cost-saving: You can save money on buying new mushroom spores every time you want to cultivate mushrooms.
– Convenience: Having spores readily available means you can cultivate mushrooms whenever you want.
– Variety: Collecting and storing spores allows you to experiment with different mushroom species.

2. How to Collect Mushroom Spores


The process of collecting mushroom spores is relatively easy and can be done at home with minimal equipment. First, select a mature mushroom with a cap that is fully opened and has started to release spores. Next, remove the stem and place the cap gill-side down on a piece of paper or a glass surface. Cover the cap with a bowl or jar and leave it for 24-48 hours. During this time, the cap will release spores onto the paper or glass surface.

Once you have collected the spores, you can store them in a suitable container.

Tools Required for Collecting Mushroom Spores:


– A mature mushroom
– A piece of paper or glass surface
– A bowl or jar to cover the mushroom cap
– Scissors or a sharp knife to remove the stem

3. Choosing the Right Container


When it comes to storing mushroom spores, choosing the right container is crucial. The container needs to be airtight, moisture-proof, and made of a non-reactive material such as glass or plastic. Using a container that is not airtight can lead to moisture buildup, which can damage the spores. Similarly, using a reactive container can cause the spores to lose viability.

Containers That Are Suitable for Storing Mushroom Spores:

| Container Type | Pros | Cons |
| — | — | — |
| Glass Vials | Airtight and easy to sterilize | Fragile and can break easily |
| Plastic Bags | Easy to use and store | Can be punctured easily |
| Glass Jars | Airtight and reusable | Can be heavy and difficult to transport |
| Petri Dishes | Sterile and suitable for laboratory use | Limited capacity |

4. Preparing the Container


Before storing mushroom spores, it is important to prepare the container properly. The container should be sterilized to ensure that there are no contaminants that can damage the spores. To sterilize the container, wash it with hot soapy water and rinse it thoroughly. Next, place the container in boiling water for 10-15 minutes. Once the container has cooled down, it is ready to be used for storing mushroom spores.

Benefits of Sterilizing the Container:


– Prevents contamination of the spores
– Increases the viability of the spores
– Ensures that the spores remain viable for longer periods

5. Filling the Container


Once you have sterilized the container, you can fill it with mushroom spores. It is important to fill the container only with the required amount of spores, leaving some headspace at the top. This headspace allows for expansion in case the spores release gas or moisture.

How Much Spores Should You Store?


It is recommended to store at least 10-20 spores per container, as this ensures that there is a higher chance of getting viable spores. However, you can store more spores if you have a larger container.

6. Sealing the Container


After filling the container with spores, it is important to seal it properly. The container should be airtight and moisture-proof to prevent any damage to the spores. You can use a sealing material such as parafilm or electrical tape to seal the container.

Benefits of Sealing the Container:


– Reduces the risk of contamination
– Prevents moisture buildup
– Increases the shelf life of the spores

7. Labeling the Container


It is important to label the container with the type of mushroom spores, the date of storage, and any other relevant information. This ensures that you can easily identify the spores when you need them and also helps to keep track of the spores’ viability.

Information That Should Be Included on the Label:


– The type of mushroom spores
– The date of storage
– Any additional information such as the source of the spores

8. Storing the Container


Mushroom spores should be stored in a cool, dry, and dark place to ensure their viability. A temperature of around 4-6°C is ideal for storing mushroom spores. You can store the container in a refrigerator or a cool, dark cupboard.

Benefits of Storing Mushroom Spores Properly:


– Ensures the viability of the spores
– Increases the shelf life of the spores
– Reduces the risk of contamination

9. Testing the Viability of the Spores


Before using the stored mushroom spores, it is important to test their viability. This can be done by inoculating a small amount of spores onto a nutrient agar plate and observing the growth of mycelium. If the spores are viable, they will germinate and develop into mycelium.

How to Test the Viability of Mushroom Spores:


– Sterilize a nutrient agar plate
– Inoculate the plate with a small amount of spores
– Incubate the plate at around 25°C for 7-10 days
– Observe the growth of mycelium

10. Using the Stored Mushroom Spores


Once you have tested the viability of the stored mushroom spores, you can use them for cultivating mushrooms. The spores can be inoculated onto a suitable substrate, such as sawdust, straw, or compost, to start the growth of mycelium. The mycelium can then be transferred to a suitable growing medium to develop into mature mushrooms.

Benefits of Using Stored Mushroom Spores:


– Cost-saving
– Convenience
– Variety

In conclusion, storing mushroom spores is a simple process that requires proper handling, sterilization, and storage conditions. By following the guidelines outlined in this guide, you can store mushroom spores for long periods and use them for cultivating mushrooms whenever you want.

Frequently Asked Questions

How long can mushroom spores be stored?

Mushroom spores can be stored for years if they are kept in the right conditions. The best way to store mushroom spores is to keep them in a cool, dry place, away from direct sunlight. If stored properly, mushroom spores can last for up to five years or even longer.

However, it is important to note that the longer the spores are stored, the lower their viability will be. So, if you are planning to store mushroom spores for a long time, it is best to take a small sample and test its viability before using it.

What is the best way to store mushroom spores?

The best way to store mushroom spores is to keep them in a sealed container that is stored in a cool and dry place, away from direct sunlight. Airtight containers such as glass jars or plastic bags can be used to store the spores.

It is also important to make sure that the container is clean and dry before storing the spores to prevent contamination. Additionally, it is recommended to store the spores in a freezer or refrigerator to extend their shelf life.

Can mushroom spores be stored in the refrigerator?

Yes, mushroom spores can be stored in the refrigerator. In fact, storing mushroom spores in the refrigerator can help to extend their shelf life. It is important to keep the spores in an airtight container to prevent moisture and contamination.

When storing mushroom spores in the refrigerator, it is recommended to keep them in the back of the refrigerator, where the temperature is the most consistent. The spores can be stored in the refrigerator for up to a year or longer.

How do you know if mushroom spores are still viable?

To test the viability of mushroom spores, you can take a small sample and grow it on a nutrient agar plate. If the spores are viable, they will germinate and grow into mycelium.

It is important to note that the viability of mushroom spores decreases over time, so it is recommended to test the spores within a few months of storage. If the spores do not germinate, it may be necessary to obtain fresh spores.

Can mushroom spores be stored in a freezer?

Yes, mushroom spores can be stored in a freezer. Freezing the spores can help to extend their shelf life, but it is important to make sure that they are stored in an airtight container to prevent moisture and contamination.

When storing mushroom spores in the freezer, it is recommended to use a vacuum-sealed bag or airtight container to prevent the spores from getting freezer burn. The spores can be stored in the freezer for up to five years or longer.

Save your spores for years!! & create a spore library!


In conclusion, storing mushroom spores can be an interesting and rewarding hobby for any enthusiast. By following the right steps, you can have a steady supply of spores to cultivate and grow your favorite mushroom varieties.

Remember to always work in a sterile environment and use the right equipment to ensure the spores stay pure and viable. Proper labelling and organization are also essential to keep track of your spore collection.

Whether you’re a seasoned mushroom grower or just starting out, storing mushroom spores is a fun and educational way to learn more about these fascinating organisms. With a little bit of knowledge and effort, you can successfully store and propagate your own mushroom spores for years to come.